How to set exposures using Zen Blue (mostly scripted version)
Автор: Cammer Michael
Загружено: 2020-10-29
Просмотров: 2698
Описание:
Problem: How to set correct exposure times for fluorescence microscopy.
Typically images are too bright with too many saturated pixels. This undermines spatial resolution and the ability to quantify relative numbers of molecules. This video explains how to set good exposures.
It assumes light fixed at 100% and imagers will only change exposure time. There is no discussion of camera gain, binning, or field size. This video exclusively addresses exposure time, resulting dynamic range, and LUT adjustments.
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Thank you Ipsita Subudhi in Shruti Naik lab at NYU Langone for sample. Imaging performed on Zeiss Imager.Z2 with a 20X PlanApoChromat N.A. 0.8 lens, X-Cite 120LED Boost light source, AxioCam 506 mono, and Zen Blue 2.6.
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