Genomic Tools for Cannabis Sativa and Psilocybe Cubensis Propagation - Kevin McKernan
Автор: Medicinal Genomics
Загружено: 2022-06-15
Просмотров: 914
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Tags: cannabis genome, cultivation, cannabis genetics, mushrooms, psilocybe, p.cubensis
Kevin McKernan presents "Genomic Tools for Cannabis Sativa and Psilocybe Cubensis Propagation" at CannMed 2022.
With many cannabis reference genomes now public, the search for pathogens that infect the plant has become greatly simplified. RNA sequencing can now be performed and screened against these genome references for novel RNA that can then be assembled and reviewed for viral content. These references greatly accelerate the development of 14 virus/viroid qPCR assays. We describe these assays’ utilization in the cannabis industry and our progress building the same genomics foundation for Psilocybe cubensis. Using Pacific Biosciences HiFi sequencing and Phase Genomics Proximity ligation assay (HiC), we constructed a chromosome-scale, annotated reference genome for Psilocybe cubensis. The genome now contains 13 chromosomes with visible telomeres on each end. Using this reference genome, we have sequenced and mapped an additional 81 Psilocybe genomes to catalog the genetic diversity and microbial contaminants frequently found in this genus. We debut a new open source, blockchain-integrated genome browser called Psilocydia.
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