Writing in DNA | How to Design CRISPR GMO Yeast
Автор: DIY Biotech
Загружено: 2022-09-13
Просмотров: 2798
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Are you ready to take on the challenge of creating cinnamon in yeast? In this video, I'll guide you through the process of designing a CRISPR GMO yeast using DNA design software, PCR, Gibson Assembly, restriction digests, and gel electrophoresis. From Yarrowia lipolytica to Saccharomyces cerevisiae, we'll cover all the steps you need to take to produce your own cinnamyl-producing yeast. Make sure to subscribe to follow along with the rest of the project!
Using CRISPR in Yarrowia with two plasmid markerless gene integration:
https://doi.org/10.1021/acssynbio.6b0...
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