Combustible Tobacco Product Cytotoxicity and Immunosuppression | Protocol Preview
Автор: JoVE (Journal of Visualized Experiments)
Загружено: 2022-07-30
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Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations - a 2 minute Preview of the Experimental Protocol
Subhashini Arimilli, Brad E. Damratoski, Prasad G.L.
Wake Forest University Health Sciences, Department of Microbiology and Immunology; R.J. Reynolds Tobacco Company, R&D Department;
Using optimized human peripheral blood mononuclear cell (PBMC) ex vivo assays, we showed that a combustible tobacco product preparation markedly suppresses receptor-mediated intracellularly secreted cytokines and cytolytic ability of effector PBMCs. These rapid assays may be useful in product evaluation and understanding the potential long-term effects of tobacco exposure.
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