Top Five Protein Estimation Methods | Preparation of Standard Curve | Dr. Nagendra Singh | PENS#36

Описание: Top Five Protein Estimation Methods | Preparation of Standard Curve | Dr. Nagendra Singh | PENS#36

#proteinestimation #bestproteinestimationmethods #standardcurve

In this video, we have discussed top five and most common methods employed for protein estimation in laboratories in a comparative manner. Preparation of standard curve and calculation of concentration is also described. These methods are:
1. UV based detection
2. Biuret method
3. Lowry method
4. BCA method
5. Bradford method
Determination of protein concentration is very necessary and widely used in protein biology, molecular biology, and other research applications in biotechnology. The concentration of protein samples need to be calculated before proceeding to isolation, purification, and analysis of a protein or enzyme. There are several protein estimation methods have been developed to quantify protein sample. Each method has its own advantages and disadvantages over others.
Three main factors should be considered before choosing a method for protein estimation.
Sensitivity- mean the the lowest concentrations method can detect.
Specificity- efficiency of the method to detect protein specifically from all other interfering substances.
Time- Duration of time for assay completion and interpretation of results.

The Lecture is given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) New Delhi. Dr. Singh has a vast experience of research and Teaching in the fields of Structure & Molecular Biology and Biochemistry, which also include Biomolecules, Proteomics, Protein Engineering and Drug Design.
Dr. Nagendra Singh currently works at the School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA (http://www.gbu.ac.in/FacultyProfiles/.... He does research in Molecular Biology, Structural Biology and Biotechnology. His current research interest include 'Structural and functional characterization of proteins involved in RNA metabolism from human and microbial origin. Dr Singh has published over 70 research articles in intentional Journals of good repute. He has over 20 awards and honors on his name. Few of the awards include "National Academy of Sciences of India (NASI, Allahabad) young scientist award", UGC-NET, CSIR-JRF, GATE, Best Research Awards etc. Dr. Singh has participated and presented research work at over 50 national and International conferences world wide.



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