Cell Disruption Techniques for Protein Prep (Beginner-Friendly Lab Tutorial)

Описание: 🧬 *In this video:*
Learn how to extract proteins from cells by combining *cell disruption (lysis)* and *centrifugation* to collect clean protein lysate for downstream analysis like Western blots or enzyme assays.

This tutorial walks through:
✔ How and why we disrupt cells to release intracellular proteins
✔ Choosing the right lysis strategy
✔ The role of centrifugation in clarifying protein extracts
✔ Tips for maximizing yield and minimizing debris
✔ Practical insights to improve your lab skills

🕒 *Timestamps:*
0:00 — Introduction & overview
0:50 — Preparing cells for disruption
2:20 — Cell lysis methods (mechanical/chemical)
4:45 — Centrifugation basics & parameters
7:10 — Collecting protein supernatant
9:00 — Review & best tips

📌 *Who this video is for:*
• Biology & biochemistry students
• Researchers preparing protein samples
• Anyone learning protein extraction techniques

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