Principles of affinity chromatography explained

Описание: Learn the principles of affinity chromatography and discover why it’s a unique and powerful method in purification technology. Unlike other techniques, affinity chromatography allows for the selective purification of biomolecules based on their biological function or specific chemical structure. This video explains the foundational concepts behind this method and its applications in research and industry.

What you’ll learn in this video:
• What is affinity chromatography? An overview of the technique and its unique capabilities.
• Key components and mechanisms involved in affinity purification.
• Practical examples of how affinity chromatography is used in biomolecule isolation.

Whether you're new to chromatography or looking to refine your understanding, this tutorial offers valuable insights into how this technique can enhance your workflows.

Explore more about the principles of affinity chromatography: https://www.cytivalifesciences.com/en...

Dive deeper into chromatography fundamentals: https://www.cytivalifesciences.com/en...

Master the basics of affinity chromatography and see how it can be a game-changer in biomolecule purification!

Chapters:
00:00:00 Introduction to affinity chromatography
00:00:05 Understanding biospecific interactions of biomolecules
00:00:09 Introduction to ligand affinity chromatography
00:00:13 Understanding the three main stages of equilibration
00:00:18 Wash and dilution process
00:00:22 Introduction to stationary phase equilibration
00:00:31 Introduction to binding process
00:00:33 Understanding target molecules and unbound material
00:00:41 Flow-through washing technique
00:00:46 Release of biomolecules in elution step
00:00:51 Understanding elution buffer composition
00:00:55 Methods to increase pH
00:01:10 Buffer and outro

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