Comet Assay for Assaying DNA damage in Neurons | Protocol Preview

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Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay - a 2 minute Preview of the Experimental Protocol

Somaira Nowsheen, Fen Xia, Eddy S. Yang
University of Alabama-Birmingham, Department of Radiation Oncology; The Ohio State University Medical School, Department of Radiation Oncology; University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham, Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center;

The comet assay is an efficient way of detecting single- and double-strand breaks, including alkali-labile sites and DNA-DNA/DNA-protein cross-links on the DNA in all cells including hippocampal neurons. The method takes advantage of the differential migration of DNA in an electric field due to differences in amount of DNA damage.

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