Rich n Chip’s Tips and Tricks for Best Quantitation and Kinetics Performance on a BLI system
Автор: Gator Bio
Загружено: 2021-11-24
Просмотров: 2110
Описание:
Biolayer interferometry (BLI) is gaining popularity for protein and small molecule quantitation and kinetics research. Even though BLI is one of the easiest of the tools, as with many other techniques, getting the best data depends on optimization of some key experimental factors. This webinar discusses, with specific examples, the impact of these factors on the results and common pitfalls.
Chapters
00:00 Introductions
01:51 About Gator Bio
05:08 Agenda Review
05:42 What is BLI?
09:15 Guidelines for all BLI Assays
10:46 Choose the right buffer
11:46 Choose the right probe
13:46 New AAVX probe
15:00 Quantitation basics
20:47 Kinetics basics
27:05 Non-Specific Binding
28:23 pM affinity interactions
30:03 Kinetics Analysis
36:04 Q&A session
#kinetics #biolayerinterferometry #tutorial
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https://en.wikipedia.org/wiki/Bio-lay...
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