Resident CD8 T Lymphocytes Imaging Using Confocal Microscopy | Protocol Preview

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Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy - a 2 minute Preview of the Experimental Protocol

Elisa Peranzoni, Houcine Bougherara, Sarah Barrin, Audrey Mansuet-Lupo, Marco Alifano, Diane Damotte, Emmanuel Donnadieu
Université Paris Descartes, Institut Cochin, Inserm U1016-CNRS UMR8104; Université Paris Descartes, Department of Pathology, Paris Centre University Hospitals; University Pierre and Marie Curie, INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center; University Paris Descartes, Department of Thoracic Surgery, Paris Centre University Hospitals;

This protocol describes a method to image resident tumor-infiltrating CD8 T cells labeled with fluorescently coupled antibodies within human lung tumor slices. This technique permits real-time analyses of CD8 T cell migration using confocal microscopy.

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