Gal4/UAS Expression for innate Immune and Cancer Cell Interactions | Protocol Preview

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Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin - a 2 minute Preview of the Experimental Protocol

Thomas Ramezani, Derek W. Laux, Isabel R. Bravo, Masazumi Tada, Yi Feng
The University of Edinburgh, MRC Centre for Inflammation Research, Queen's Medical Research Institute; University College London, Department of Cell & Developmental Biology;

Studying the earliest events of preneoplastic cell progression and innate immune cell interaction is pivotal to understand and treat cancer. Here we describe a method to conditionally induce epithelial cell transformations and the subsequent live imaging of innate immune cell interaction with HRASG12V expressing skin cells in zebrafish larvae.

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