Bacterial Transformation Lab (Theory)
Автор: Stephen Borish
Загружено: 2017-04-12
Просмотров: 57789
Описание:
In this animation produced by WGBH and Digizyme, Inc., learn about bacterial transformation, the process by which DNA plasmids are introduced into a bacterial cell's genome. The animation presents the physical challenges of getting the plasmids through the cell's plasma membrane and the "heat shock" technique used to overcome them. It also shows how researchers test for successful transformations using experimental and negative control plates.
1. What is bacterial transformation?
2. Explain the primary physical obstacle to transforming bacteria in an experimental setting.
3. What is the purpose of exposing the bacterial cell and plasmid mixture to cold and then hot temperatures?
4. Explain the experimental setup involving the culture plates.
5. Describe what you should observe in each of the three plates by the end of this phase of the experiment, and why.
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