Targeted DNA Methylation Analysis: Next-Generation Sequencing -Preview

Описание: Watch the Full Video at https://www.jove.com/v/52488/targeted....

Targeted DNA Methylation Analysis by Next-generation Sequencing - a 2 minute Preview of the Experimental Protocol

Dustin R. Masser, David R. Stanford, Willard M. Freeman
University of Oklahoma College of Medicine, Department of Physiology; University of Oklahoma College of Medicine, Department of Geriatric Medicine;

Bisulfite amplicon sequencing (BSAS) is a method for quantifying cytosine methylation in targeted genomic regions of interest. This method uses bisulfite conversion paired with PCR amplification of target regions prior to next-generation sequencing to produce absolute quantitation of DNA methylation at a base-specific level.

Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel:    / jovejournalofvisualizedexperiments