M2 - MAIV on SYNAPT G2
Автор: Milan Pophristic
Загружено: 2016-06-14
Просмотров: 243
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Vacuum MAI (MAIV) on SYNAPT G2
The manufacturer’s intermediate pressure MALDI housing was modified by removing the hexapole ion guide and replacing the sample introduction module with a vacuum lock for a solid probe.
Because the vacuum lock does not require pumping, it can be quickly opened to vacuum so that matrix:analyte samples placed on the flat end of a metal probe are rapidly inserted into the MALDI housing assembly where ionization occurs spontaneously.
The MAI samples were prepared by 1:1 mixing of analyte in water and 0.006g/ml 3-nitrobenzonitrile (NBN) matrix in 7:1 acetonitrile :water.
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