Assessment of Select Compounds on In Vitro Ruminal Fermentation ... - Alice Peres Assumpção

Описание: 2025 Dairy Symposium hosted by UW–Madison:

Assessment of select compounds on in vitro ruminal fermentation and their potential for methane mitigation” – Alice Peres Assumpção, animal and dairy sciences, UW–Madison

Assumpção is pursuing a PhD in animal and dairy sciences and is mentored by Hilario Mantovani. She earned her master’s in animal productivity and quality from University of Sao Paulo in Brazil. Her research project evaluated nutritional strategies to mitigate the methane emission on beef cattle production, through evaluation of the parameters and products of enteric fermentation and the rumen microbiota.

Project summary: Many strategies to mitigate CH4 emissions and enhance nutrient utilization have been proposed, including the use of feed additives. The current work aimed to explore the anti-methanogenic capacity of pure compounds for potential development of new additives with a greater potential to minimize methanogenesis with minimum side effects. Four in vitro experiments explored gas production and composition, VFAs, rumen microbiota, and pH. The first experiment screened 16 molecules, including polyphenols, flavonoids, fatty acids, amino acids, and salts, at 10 mM. Seven molecules advanced to the second experiment, which included four nitro compounds with known anti-methanogenic properties. Clustering analyses identified four promising compounds: carvacrol, nitroethane, 2-nitroethanol, and 2-nitro-1-propanol. These were tested at concentrations of 0, 1.25, 2.5, 5, and 10 mM to determine their effectiveness. The final experiment examined the synergistic effects of carvacrol and 2-nitroethanol in a full dose and half dose, suggesting potential for enhanced methane reduction strategies.

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