Gene Set Enrichment Analysis (GSEA) | Pluto Bio
Автор: Pluto Bio
Загружено: 2025-01-04
Просмотров: 711
Описание:
Gene set enrichment analysis (GSEA) is a common approach to performing pathway analysis on differential expression analysis results, but there is some nuance to interpreting the results. Here, we do a deep-dive into how to perform GSEA and how to interpret its visualizations using Pluto.
0:12 Introduction to GSEA on the Pluto canvas
1:08 Overview of the RNA-seq experiment being used for this example
2:48 Creating a gene set enrichment analysis
3:20 Adding a GSEA widget to your canvas in Pluto
3:54 Setting up your GSEA parameters
4:11 Introduction to the MSigDB gene set collections
4:51 Introduction to an enrichment plot and selecting a gene set to display
5:36 Interpreting a GSEA enrichment plot
6:56 Customizing enrichment plot appearance
8:24 Connecting GSEA enrichment plot back to a volcano plot
9:40 Using a barplot to show multiple pathways' scores
11:14 Customizing barplot appearance
11:46 Viewing publication-ready analysis Methods
Thanks for watching! Feel free to schedule a call with our scientists to see how Pluto can be used for your own data analysis: https://pluto.bio/get-info
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