SGD On Gram Staining: Principle, Procedure, and Results Explained | MBBS

Описание: This comprehensive lecture and practical demonstration, delivered to the MBBS (2024-2025) batch at Santiniketan Medical College & Hospital, breaks down everything you need to know. Master the principles, learn the step-by-step procedure, and understand the core difference between Gram Positive (Purple/Violet) and Gram Negative (Pink) bacteria based on their cell wall structure. This is a must-watch for all microbiology students and clinical trainees!

Keywords Targeted: Gram Staining, Gram Stain Principle, Gram Staining Procedure, MBBS Practical, Microbiology Lab, Differential Staining, Peptidoglycan Layer, Gram Positive, Gram Negative, Medical Lecture.
Video Timestamps & Key Topics

Use this time-stamped guide to navigate through the lecture and focus on revision points:
Timestamp Topic Covered
[00:00] Introduction: Why We Do Staining (Visibility, Contrast, Identification)
[01:15] Identifying Bacterial Morphology (Shape, Size, Arrangement, e.g., Bacillus, Coccus)
[02:21] Required Reagents (Stains, Mordant, Decolorizer, Contrast)
[04:45] Types of Staining: Simple vs. Differential Staining
[06:36] Who Discovered Gram Staining? (Hans Christian Gram)
[08:33] Practical Setup: Smear Preparation (Cleaning Slide, Normal Saline, Inoculation, Heat Fixation)
[10:07] Step 1: Primary Stain (Crystal Violet for 1 minute)
[10:37] Step 2: Mordant (Gram's Iodine for 1 minute)
[10:51] Step 3: Decolorization (Acetone/Alcohol - Tilt and quick wash)
[11:29] Step 4: Counterstain (Safranin)
[11:36] Final Results: Gram Positive (Violet/Purple) vs. Gram Negative (Pink)
[12:07] Principle of Gram Staining (Relies on Two Core Theories)
[12:30] Theory 1: pH Theory (Cytoplasm Acidity and Charge)
[13:25] Theory 2: Cell Wall Theory (Thick vs. Thin Peptidoglycan Layer)
[14:45] Student Practical Demonstration Begins
[26:26] Viewing the Slide under Oil Immersion (100x)
[29:06] Live Identification of Gram Negative (Pink Rods) and Gram Positive


SANTINIKETAN MEDICAL COLLEGE & HOSPITAL
Real Classroom Demonstration/Lecture
On: MICROBIOLOGY by Dr. Himanshu Singh Bisht
(MBBS Batch: 2024-25)
Conducted on: 05-12-25