DNA Stable-Isotope Probing (DNA-SIP) l Protocol Preview
Автор: JoVE (Journal of Visualized Experiments)
Загружено: 2022-06-03
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DNA Stable-Isotope Probing (DNA-SIP) - a 2 minute Preview of the Experimental Protocol
Eric A. Dunford, Josh D. Neufeld
University of Waterloo, Department of Biology;
DNA stable-isotope probing is a cultivation-independent method to identify and characterize active communities of microorganisms that are capable of utilizing specific substrates. Assimilation of substrate enriched in heavy isotope leads to incorporation of labelled atoms into microbial biomass. Density gradient ultracentrifugation retrieves labelled DNA for downstream molecular analyses.
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