Biomass Separation Strategies in Microbial Fermentation

Описание: In this episode we focus on the technical criteria for selecting biomass separation strategies in microbial fermentation, focusing on how cell morphology, broth rheology, and product localization dictate industrial success. It explains that bacterial systems often require centrifugation due to their small size and tendency to form compressible cakes, though this carries a risk of shear-induced lysis. In contrast, filamentous fungal processes rely on morphological control to manage high viscosity and ensure efficient filtration. The discussion further highlight how extracellular polymers and solids load act as critical variables that can cause membrane fouling or hydraulic failure. Ultimately, the overview emphasizes that a robust harvest strategy must balance throughput requirements with the need to minimize impurity release based on whether the desired product is intracellular or extracellular.

#Bioprocess #ScaleUp and #TechTransfer,#Industrial #Microbiology,#MetabolicEngineering and #SystemsBiology,#Bioprocessing,#MicrobialFermentation,#Bio-manufacturing,#Industrial #Biotechnology,#Fermentation Engineering,#ProcessDevelopment,#Microbiology,#Biochemistry,#Biochemical Engineering, #Applied #MicrobialPhysiology, #Microbial #ProcessEngineering, #Upstream #BioprocessDevelopment, #Downstream Processing and #Purification,#CellCulture and #MicrobialSystems Engineering, #Bioreaction #Enzymes, #Biocatalyst #scientific #Scientist #Research
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Timestamp
Timestamp Problem Addressed
02:44–04:15 The Oxygen Transfer Rate (OTR) Bottleneck.
05:30–07:05 Gradient Formation & $t_{mix}$ vs. $t_{bio}$.
08:20–10:00 Shear Stress and the Kolmogorov Scale.
11:45–13:15 Feed-Zone Overflow Metabolism.
14:30–15:45 The Scale-Down Mimic Strategy.