High Yield Expression Of Recombinant Human Proteins: Transient Transfection l Protocol Preview

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High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension - a 2 minute Preview of the Experimental Protocol

Ganesh P. Subedi, Roy W. Johnson, Heather A. Moniz, Kelley W. Moremen, Adam Barb
Iowa State University, The Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology; University of Georgia, Complex Carbohydrate Research Center;

Laboratory-scale production of eukaryotic proteins with appropriate post-translational modification represents a significant barrier. Here is a robust protocol with rapid establishment and turnaround for protein expression using a mammalian expression system. This system supports selective amino acid, selective labeling of proteins and small molecule modulators of glycan composition.

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