MaxWell Webinar - Morphological, functional correlation of retinal organoids to the human retina

Описание: Replay from February 18, 2021

Host: Dr. Marie Obien, VP Sales & Marketing at MaxWell Biosystems

Speakers:
Martina de Gennaro, Doctoral Researcher at Institute of Molecular and Clinical Ophtalmology Basel (IOB)
Dr. Szilard Sajgo, Application Scientist at MaxWell Biosystems


Genetic disorders of the human retina cause visual impairment to millions of people worldwide. The retina is a well characterized tissue at the back of the eye, and is a potential target for visual restoration therapies. Light-sensitive human retinal organoids recapitulate cell-types, circuitry and transcriptomic profiles of the human retina, offering a relevant tool for translational studies.

In this webinar, Dr. Szilárd Sajgó, our R&D scientist and an expert in retina research will:

Introduce the different analyses of retinal function that can be performed using high-density microelecrode arrays (HD-MEAs),
Explain how the efficacy of visual restoration therapies can now be easily assessed using HD-MEAs.
This is followed by Ms. Martina De Gennaro, Doctoral Researcher at Institute of Molecular and Clinical Ophtalmology Basel (IOB) and co-author of this recent Cell paper, who will:

Demonstrate how the generation of a library of 285,441 single-cell transcriptomes from light-responsive human retinae and retinal organoids at different time points allows the comparison of developmental rates and genomic expression profiles,
Explain how electrophysiological assessments, such as HD-MEA and calcium imaging, ensure the collection of transcriptomes from functional, light-responsive human retinae ex-vivo,
Discuss how this research is allowing to define cellular targets for disease mechanisms investigation in organoids and targeted repair in the human retina.