Human Pluripotent and Neural Stem cells culture | Protocol Preview
Автор: JoVE (Journal of Visualized Experiments)
Загружено: 2022-08-23
Просмотров: 589
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Watch the Full Video at https://www.jove.com/v/53685/culturin....
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research - a 2 minute Preview of the Experimental Protocol
Alexander E. Stover, Siranush Herculian, Maria G. Banuelos, Samantha L. Navarro, Michael P. Jenkins, Philip H. Schwartz
Childrens Hospital of Orange County Research Institute, National Human Neural Stem Cell Resource;
Here is a protocol to grow pluripotent stem cells (PSC) and neural stem cells (NSC) in an enclosed cell culture system that permits maximum sterility and reproducibility, replacing the traditional biosafety cabinet and incubator. This equipment meets clinical good manufacturing practice (cGMP) and clinical good lab practice (cGLP) guidelines.
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