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2nd Centrifugation (GFP Recovery part 4 of 5)
Автор: BioNetwork
Загружено: 2014-08-05
Просмотров: 6552
Описание: After the second homogenization, the lysed cell solution is pumped back through the centrifuge. But this time, our goal is different. Before lysing, our product was held within the E.coli cells. Now – with the cells broken apart, the cell contents - including cytoplasm and GFP - are mixed into the buffered solution. The centrifuge again spins out the solids - which are primarily cell debris - and it’s the clarified liquid which contains our product – the GFP! This time we discard the solids and keep the liquid – which is now known as Lysate.
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