mRNA Transcripts Stability Identification by Plasmid Expression System | Protocol Preview

Описание: Watch the Full Video at https://www.jove.com/v/60654/efficien....

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells - a 2 minute Preview of the Experimental Protocol

Francis Migneault, Frédéric Gagnon, Emmanuelle Brochiero, Yves Berthiaume, André Dagenais
Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM),; Institut de Recherches Cliniques de Montréal (IRCM),; Université de Montréal, Département de médecine, Faculté de médecine;

Here, we present a tool that can be used to study the posttranscriptional modulation of a transcript in primary alveolar epithelial cells by using an inducible expression system coupled to a pipette electroporation technique.

Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel:    / jovejournalofvisualizedexperiments