Translating Ribosome Affinity Purification-Rare Cell Populations: Drosophila l Protocol Preview

Описание: Watch the Full Video at https://www.jove.com/v/52985/trap-rc-....

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos - a 2 minute Preview of the Experimental Protocol

Benjamin Bertin, Yoan Renaud, Rajaguru Aradhya, Krzysztof Jagla, Guillaume Junion
University of Clermont-Ferrand,; Memorial Sloan Kettering Cancer Center,;

Translating Ribosome Affinity Purification (TRAP) is able to capture cell-type-specific translation of mRNA. Here we report the first TRAP protocol dedicated to isolation of mRNA in rare cell populations of Drosophila embryos.

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