Tips & Tricks for Successful SignalStar® Multiplex Experiments

Описание: Before you run your first SignalStar® Multiplex IHC Assay, get the insider knowledge directly from the CST spatial biology experts who design and validate SignalStar products and protocols. This video highlights key sections of the protocol, and will help you to:

• Understand critical steps in the SignalStar protocol
• Avoid common experimental pitfalls
• Maximize experimental success for both manual and automated SignalStar staining protocols
• Be confident in your results

Related resources:

SignalStar Multiplex IHC Resource Page: https://www.cellsignal.com/applicatio...
SignalStar Multiplex IHC Frequently Asked Questions: https://www.cellsignal.com/applicatio...
SignalStar Multiplex IHC Troubleshooting: https://www.cellsignal.com/applicatio...
How to Perform Antigen Retrieval (AR) in Immunohistochemistry (IHC):    • How to perform Antigen Retrieval (AR) in I...  
Step-by-Step Instructions for Multiplex IHC Image Coregistration with QuPath: https://learn.cellsignal.com/signalst...
Contact Technical Support: https://www.cellsignal.com/support

Video chapters:
0:00 Who this video is for
0:23 Introduction
2:03 Sample Compatibility
2:28 Working with Viscous Solutions (Most important tip!)
4:10 Expected Signal Brightness
4:53 Image Alignment/Coregistration
5:59 Specific Tips for Manual SignalStar IHC
8:14 Specific Tips for Automated SignalStar IHC

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