A Novel Approach for dsRNA Removal Using Oligo dT Affinity Chromatography by Targeting Hydrogen Bond

Описание: Join Rok Sekirnik in this BioProcess International Ask the Expert webcast as he delves into the latest advancements in mRNA vaccine and therapeutic production. Discover innovative methods for tackling the challenge of immunogenic double-stranded RNA (dsRNA) that arises during in vitro transcription (IVT) reactions. Learn how Sartorius BIA Separations has pioneered a novel technique to destabilize dsRNA by targeting its hydrogen bonds, crucial for its stability. This groundbreaking method uses pH modulation to achieve controlled denaturation in a safe manner, followed by purification using Oligo dT affinity chromatography. Gain insights into how this scalable, high-recovery approach enhances mRNA integrity, reduces immunogenicity, and elevates the overall quality, safety, and manufacturability of mRNA products. Whether you're working in gene therapy, vaccine development, or RNA purification, this session provides valuable knowledge to optimize your RNA production processes.

Key Takeaways:
dsRNA stability depends on hydrogen bonding.
Disrupting hydrogen bonding destabilizes dsRNA into individual RNA strands. Target mRNA is selectively isolated with affinity Oligo dT chromatography.
This approach is scalable, high-recovery (more than 90%), and GMP compliant.

Speaker:
Rok Sekirnik, Head Process Development mRNA | pDNA, Sartorius BIA Separations

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