How to use Invitrogen LIVE/DEAD Cell Imaging Kit with P3D Scaffolds
Автор: Ossiform
Загружено: 2022-12-20
Просмотров: 7081
Описание:
NOTE: Error in captions at 01:22. You must add 1mL green staining solution to the 1uL red staining solution.
Video protocol for how to perform Cell viability using Invitrogen LIVE/DEAD Cell Imaging Kit with P3D Scaffolds from @Ossiform Research Line.
Note: This video is made with customized P3D Scaffolds.
Content:
00:13 - Thaw the cell kit at room temperature
00:43 - Aspirate the media from the scaffold
01:09 - Add 200uL media to each scaffold
01:22 - Mix the live/dead cell kit solution by adding 1mL green staining solution to the 1uL red staining solution
02:06 - Add 200uL from the live/dead cell kit solution on the top of each scaffold
02:45 - Cover with tinfoil to prevent it from the light damage and incubate for 15 min. at room temperature
02:59 - Flip each scaffold if the microscope's objective is located at the bottom site of the specimen
03:17 - Evaluate cell viability using flourescent microscope
Make sure to check our latest updated protocols on our website:
https://ossiform.com/ossiform-researc...
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